DBNascent | Samples

SRR8950260 Sample ID 2785

**Data sources**
Paper identifier	Zhang2019fundamental
SRP accession	SRP193758
GEO accession	GSE130266
Samples	SRR8950260

**Technical details**
QC score	3
Replicate	2
Protocol	GRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	paired
Raw strandedness	read1 forward
Reverse complemented?	True
Mapped strandedness	read1 forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	167 Mb
Stranded gene read counts	TXT file	927 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	2 Mb
dREG bidirectional regions	BED file	863 Kb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	primary cell
Cell type	splenic B cell
Clone/Individual
Strain	C57BL/6
Genotype	Aid -/-
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	anti-CD40 (1 microg/mL)	-48 hr	0 hr	2 day
treatment	interleukin-4 (20 ng/mL)	-48 hr	0 hr	2 day

**Extended notes**
Pipeline info and software versions
Sample notes
Analysis notes	Unstranded; Ran qc redo nextflow

**Sample metrics**
Sample QC score	3
Sample NRO score	1
Raw read length	76
Read depth after trimming	85,406,640
Duplication proportion	0.69375
Proportion reads mapped	0.6969
Exon/intron ratio	2.59249
Unique read proportion	0.53938

**Bidirectionals**
Total # Tfit bidirectionals	26881
# Promoter Tfit bidirectionals	7546
# Intronic Tfit bidirectionals	10651
# Intergenic Tfit bidirectionals	4755
Total # dREG bidirectionals	19792
# Promoter dREG bidirectionals	4686
# Intronic dREG bidirectionals	7883
# Intergenic dREG bidirectionals	4355
Part of Tfit master merge?	False
Part of dREG master merge?	False