DBNascent | Samples

SRR8950261 Sample ID 2786

**Data sources**
Paper identifier	Zhang2019fundamental
SRP accession	SRP193758
GEO accession	GSE130266
Samples	SRR8950261

**Technical details**
QC score	1
Replicate	3
Protocol	GRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	paired
Raw strandedness	read1 forward
Reverse complemented?	True
Mapped strandedness	read1 forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	201 Mb
Stranded gene read counts	TXT file	920 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	3 Mb
dREG bidirectional regions	BED file	925 Kb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	primary cell
Cell type	splenic B cell
Clone/Individual
Strain	C57BL/6
Genotype	Aid -/-
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	anti-CD40 (1 microg/mL)	-48 hr	0 hr	2 day
treatment	interleukin-4 (20 ng/mL)	-48 hr	0 hr	2 day

**Extended notes**
Pipeline info and software versions
Sample notes
Analysis notes	Unstranded; Ran qc redo nextflow

**Sample metrics**
Sample QC score	1
Sample NRO score	1
Raw read length	76
Read depth after trimming	40,057,615
Duplication proportion	0.2612
Proportion reads mapped	0.8189
Exon/intron ratio	1.82914
Unique read proportion	0.69611

**Bidirectionals**
Total # Tfit bidirectionals	30644
# Promoter Tfit bidirectionals	7706
# Intronic Tfit bidirectionals	13275
# Intergenic Tfit bidirectionals	5998
Total # dREG bidirectionals	21230
# Promoter dREG bidirectionals	4711
# Intronic dREG bidirectionals	9275
# Intergenic dREG bidirectionals	5055
Part of Tfit master merge?	False
Part of dREG master merge?	False