DBNascent | Samples

SRR8957138 Sample ID 274

**Data sources**
Paper identifier	Birkenheuer2020rna
SRP accession	SRP193891
GEO accession	GSE130342
Samples	SRR8957138

**Technical details**
QC score	1
Replicate	3
Protocol	PRO-seq
Library prep	ligation
Spike-in	D. melanogaster
Single/Paired end	single
Raw strandedness	reverse
Reverse complemented?	True
Mapped strandedness	forward
Timecourse	True
Control/Experimental	control
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	102 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	3 Mb
dREG bidirectional regions	BED file	941 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	HEp2
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	HSV-1 (MOI 5)	-6 hr	-5 hr	1 hr

**Extended notes**
Pipeline info and software versions
Sample notes
Analysis notes

**Sample metrics**
Sample QC score	1
Sample NRO score	2
Raw read length	75
Read depth after trimming	43,683,688
Duplication proportion	0.49299
Proportion reads mapped	0.6144
Exon/intron ratio	4.21363
Unique read proportion	0.60077

**Bidirectionals**
Total # Tfit bidirectionals	33028
# Promoter Tfit bidirectionals	13628
# Intronic Tfit bidirectionals	13266
# Intergenic Tfit bidirectionals	6030
Total # dREG bidirectionals	21530
# Promoter dREG bidirectionals	8215
# Intronic dREG bidirectionals	8819
# Intergenic dREG bidirectionals	4144
Part of Tfit master merge?	True
Part of dREG master merge?	True