DBNascent | Samples

SRR8957139 Sample ID 275

**Data sources**
Paper identifier	Birkenheuer2020rna
SRP accession	SRP193891
GEO accession	GSE130342
Samples	SRR8957139

**Technical details**
QC score	1
Replicate	1
Protocol	PRO-seq
Library prep	ligation
Spike-in	D. melanogaster
Single/Paired end	single
Raw strandedness	reverse
Reverse complemented?	True
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	119 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	3 Mb
dREG bidirectional regions	BED file	1 Mb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	HEp2
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	HSV-1 (MOI 5)	-3 hr	-2 hr	1 hr
treatment	cycloheximide (10 microM)	-4 hr	0 hr	4 hr

**Extended notes**
Pipeline info and software versions
Sample notes
Analysis notes

**Sample metrics**
Sample QC score	1
Sample NRO score	2
Raw read length	76
Read depth after trimming	35,890,733
Duplication proportion	0.39414
Proportion reads mapped	0.8296
Exon/intron ratio	4.6197
Unique read proportion	0.67422

**Bidirectionals**
Total # Tfit bidirectionals	35772
# Promoter Tfit bidirectionals	13732
# Intronic Tfit bidirectionals	14502
# Intergenic Tfit bidirectionals	8043
Total # dREG bidirectionals	26723
# Promoter dREG bidirectionals	6992
# Intronic dREG bidirectionals	12242
# Intergenic dREG bidirectionals	6271
Part of Tfit master merge?	True
Part of dREG master merge?	True