DBNascent | Samples

SRR8957143 Sample ID 279

**Data sources**
Paper identifier	Birkenheuer2020rna
SRP accession	SRP193891
GEO accession	GSE130342
Samples	SRR8957143

**Technical details**
QC score	2
Replicate	2
Protocol	PRO-seq
Library prep	ligation
Spike-in	D. melanogaster
Single/Paired end	single
Raw strandedness	reverse
Reverse complemented?	True
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	145 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	4 Mb
dREG bidirectional regions	BED file	1 Mb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	HEp2
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	HSV-1 (MOI 5)	-6 hr	-5 hr	1 hr
treatment	phosphonoacetic acid (200 microg/mL)	-6 hr	0 hr	6 hr

**Extended notes**
Pipeline info and software versions
Sample notes
Analysis notes

**Sample metrics**
Sample QC score	2
Sample NRO score	2
Raw read length	75
Read depth after trimming	52,176,855
Duplication proportion	0.5528
Proportion reads mapped	0.8573
Exon/intron ratio	4.53231
Unique read proportion	0.59096

**Bidirectionals**
Total # Tfit bidirectionals	40605
# Promoter Tfit bidirectionals	15276
# Intronic Tfit bidirectionals	15778
# Intergenic Tfit bidirectionals	9062
Total # dREG bidirectionals	26129
# Promoter dREG bidirectionals	7961
# Intronic dREG bidirectionals	11325
# Intergenic dREG bidirectionals	5989
Part of Tfit master merge?	True
Part of dREG master merge?	True