DBNascent | Samples

SRR9032554 Sample ID 2332

**Data sources**
Paper identifier	Steinparzer2019transcriptional
SRP accession	SRP197255
GEO accession
Samples	SRR9032554

**Technical details**
QC score	3
Replicate	1
Protocol	GRO-seq
Library prep	random primed
Spike-in	None
Single/Paired end	single
Raw strandedness	reverse
Reverse complemented?	True
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	85 Mb
Stranded gene read counts	TXT file	920 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	2 Mb
dREG bidirectional regions	BED file	797 Kb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	cell line
Cell type	MEF
Clone/Individual
Strain	C57BL/6
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	IFN-gamma (10 ng/mL)	-30 min	0 min	30 min
treatment	cortistatin A (100 nM)	-90 min	-30 min	1 hr

**Extended notes**
Pipeline info and software versions
Sample notes
Analysis notes

**Sample metrics**
Sample QC score	3
Sample NRO score	2
Raw read length	151
Read depth after trimming	84,086,227
Duplication proportion	0.73492
Proportion reads mapped	0.5584
Exon/intron ratio	3.9802
Unique read proportion	0.39115

**Bidirectionals**
Total # Tfit bidirectionals	20757
# Promoter Tfit bidirectionals	6581
# Intronic Tfit bidirectionals	7662
# Intergenic Tfit bidirectionals	4025
Total # dREG bidirectionals	18006
# Promoter dREG bidirectionals	4221
# Intronic dREG bidirectionals	7015
# Intergenic dREG bidirectionals	3378
Part of Tfit master merge?	True
Part of dREG master merge?	True