DBNascent | Samples

SRR915734 Sample ID 1434

**Data sources**
Paper identifier	Le2013mapping
SRP accession	SRP026204
GEO accession	GSE48159
Samples	SRR915734

**Technical details**
QC score	3
Replicate	2
Protocol	GRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	59 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	1 Mb
dREG bidirectional regions	BED file	402 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	MCF7
Clone/Individual	clone C4-12
Strain
Genotype	ER-beta flag (stable)
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	17-beta-estradiol (10 nM)	-1 hr	0 hr	1 hr

**Extended notes**
Pipeline info and software versions
Sample notes	MCF7 C4-12 clonal line has been propagated to lose expression of ERalpha (not sure if specific mutations or epigenetically)
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	3
Sample NRO score	1
Raw read length	51
Read depth after trimming	51,830,478
Duplication proportion	0.78605
Proportion reads mapped	0.5071
Exon/intron ratio	2.25794
Unique read proportion	0.39905

**Bidirectionals**
Total # Tfit bidirectionals	15044
# Promoter Tfit bidirectionals	4913
# Intronic Tfit bidirectionals	7691
# Intergenic Tfit bidirectionals	1453
Total # dREG bidirectionals	9358
# Promoter dREG bidirectionals	5314
# Intronic dREG bidirectionals	3245
# Intergenic dREG bidirectionals	1022
Part of Tfit master merge?	True
Part of dREG master merge?	True