DBNascent | Samples

SRR929980 Sample ID 2522

**Data sources**
Paper identifier	Veloso2013genome-wide
SRP accession	SRP026690
GEO accession	GSE48678
Samples	SRR929980

**Technical details**
QC score	3
Replicate	1
Protocol	Bru-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	reverse
Reverse complemented?	True
Mapped strandedness	forward
Timecourse	True
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	102 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	414 Kb
dREG bidirectional regions	BED file	251 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	HF1
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	bromouridine (2 mM)	-15 min	0 min	15 min
treatment	camptothecin (20 microM)	-90 min	-30 min	1 hr

**Extended notes**
Pipeline info and software versions
Sample notes	BrU-seq
Analysis notes

**Sample metrics**
Sample QC score	3
Sample NRO score	1
Raw read length	52
Read depth after trimming	58,737,461
Duplication proportion	0.74301
Proportion reads mapped	0.9718
Exon/intron ratio	2.26667
Unique read proportion	0.52437

**Bidirectionals**
Total # Tfit bidirectionals	4713
# Promoter Tfit bidirectionals	1191
# Intronic Tfit bidirectionals	2641
# Intergenic Tfit bidirectionals	368
Total # dREG bidirectionals	5803
# Promoter dREG bidirectionals	1606
# Intronic dREG bidirectionals	3265
# Intergenic dREG bidirectionals	677
Part of Tfit master merge?	True
Part of dREG master merge?	True