DBNascent | Samples

SRR929983 Sample ID 2523

**Data sources**
Paper identifier	Veloso2013genome-wide
SRP accession	SRP026690
GEO accession	GSE48678
Samples	SRR929983

**Technical details**
QC score	4
Replicate	1
Protocol	Bru-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	reverse
Reverse complemented?	True
Mapped strandedness	forward
Timecourse	True
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	60 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	370 Kb
dREG bidirectional regions	BED file	179 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	primary cell
Cell type	skin fibroblast
Clone/Individual	GM00739
Strain
Genotype	Csb CS1AN mut
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	bromouridine (2 mM)	-15 min	0 min	15 min
treatment	camptothecin (20 microM)	-60 min	0 min	1 hr

**Extended notes**
Pipeline info and software versions
Sample notes	BrU-seq; Cockayne syndrome fibroblasts (GM00739)
Analysis notes

**Sample metrics**
Sample QC score	4
Sample NRO score	2
Raw read length	51
Read depth after trimming	75,076,807
Duplication proportion	0.90093
Proportion reads mapped	0.9713
Exon/intron ratio	4.6009
Unique read proportion	0.22688

**Bidirectionals**
Total # Tfit bidirectionals	4226
# Promoter Tfit bidirectionals	716
# Intronic Tfit bidirectionals	2339
# Intergenic Tfit bidirectionals	148
Total # dREG bidirectionals	4133
# Promoter dREG bidirectionals	1116
# Intronic dREG bidirectionals	2086
# Intergenic dREG bidirectionals	507
Part of Tfit master merge?	True
Part of dREG master merge?	True