DBNascent | Samples

SRR929985 Sample ID 2525

**Data sources**
Paper identifier	Veloso2013genome-wide
SRP accession	SRP026690
GEO accession	GSE48678
Samples	SRR929985

**Technical details**
QC score	4
Replicate	1
Protocol	Bru-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	reverse
Reverse complemented?	True
Mapped strandedness	forward
Timecourse	True
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	72 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	248 Kb
dREG bidirectional regions	BED file	131 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	primary cell
Cell type	skin fibroblast
Clone/Individual	GM00739
Strain
Genotype	Csb CS1AN mut
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	bromouridine (2 mM)	-15 min	0 min	15 min
treatment	camptothecin (20 microM)	-90 min	-30 min	1 hr

**Extended notes**
Pipeline info and software versions
Sample notes	BrU-seq; Cockayne syndrome fibroblasts (GM00739)
Analysis notes

**Sample metrics**
Sample QC score	4
Sample NRO score	2
Raw read length	51
Read depth after trimming	58,452,705
Duplication proportion	0.83183
Proportion reads mapped	0.9735
Exon/intron ratio	2.98817
Unique read proportion	0.28532

**Bidirectionals**
Total # Tfit bidirectionals	2826
# Promoter Tfit bidirectionals	415
# Intronic Tfit bidirectionals	1497
# Intergenic Tfit bidirectionals	196
Total # dREG bidirectionals	3023
# Promoter dREG bidirectionals	654
# Intronic dREG bidirectionals	1539
# Intergenic dREG bidirectionals	520
Part of Tfit master merge?	True
Part of dREG master merge?	True