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SRR9304731 Sample ID 767

**Data sources**
Paper identifier	Fant2020tfiid
SRP accession	SRP201480
GEO accession	GSE132764
Samples	SRR9304731

**Technical details**
QC score	2
Replicate	2
Protocol	PRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	reverse
Reverse complemented?	True
Mapped strandedness	forward
Timecourse	False
Control/Experimental	control
Wildtype & Untreated?	True
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	357 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	3 Mb
dREG bidirectional regions	BED file	2 Mb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	HCT116
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	mock treatment	-1 hr	0 hr	1 hr

**Extended notes**
Pipeline info and software versions
Sample notes	Mock treatment means cells exposed to same electroporation pulse as treated cells; Cites Mahat2016mammalian for protocol and library prep
Analysis notes

**Sample metrics**
Sample QC score	2
Sample NRO score	2
Raw read length	76
Read depth after trimming	132,287,922
Duplication proportion	0.56664
Proportion reads mapped	0.9545
Exon/intron ratio	3.33253
Unique read proportion	0.68298

**Bidirectionals**
Total # Tfit bidirectionals	38880
# Promoter Tfit bidirectionals	14602
# Intronic Tfit bidirectionals	14150
# Intergenic Tfit bidirectionals	9377
Total # dREG bidirectionals	38933
# Promoter dREG bidirectionals	7063
# Intronic dREG bidirectionals	18237
# Intergenic dREG bidirectionals	11369
Part of Tfit master merge?	False
Part of dREG master merge?	False