DBNascent | Samples

SRR930673 Sample ID 1141

**Data sources**
Paper identifier	Kaikkonen2013remodeling
SRP accession	SRP026695
GEO accession	GSE48759
Samples	SRR930673

**Technical details**
QC score	3
Replicate	2
Protocol	GRO-seq
Library prep	circularization
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	True
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	48 Mb
Stranded gene read counts	TXT file	909 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	1 Mb
dREG bidirectional regions	BED file	523 Kb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	primary cell
Cell type	peritoneal macrophage
Clone/Individual
Strain	C57BL/6
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	Kdo2-Lipid A (100 ng/mL)	-12 hr	0 hr	12 hr
treatment	serum starvation (0.5% FBS)	-26 hr	0 hr	26 hr

**Extended notes**
Pipeline info and software versions
Sample notes	Replicate information unclear
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	3
Sample NRO score	2
Raw read length	53
Read depth after trimming	14,782,247
Duplication proportion	0.42695
Proportion reads mapped	0.8584
Exon/intron ratio	3.43322
Unique read proportion	0.59234

**Bidirectionals**
Total # Tfit bidirectionals	16278
# Promoter Tfit bidirectionals	7211
# Intronic Tfit bidirectionals	5007
# Intergenic Tfit bidirectionals	2526
Total # dREG bidirectionals	12110
# Promoter dREG bidirectionals	5278
# Intronic dREG bidirectionals	3464
# Intergenic dREG bidirectionals	2078
Part of Tfit master merge?	True
Part of dREG master merge?	True