DBNascent | Samples

SRR930674 Sample ID 1142

**Data sources**
Paper identifier	Kaikkonen2013remodeling
SRP accession	SRP026695
GEO accession	GSE48759
Samples	SRR930674

**Technical details**
QC score	3
Replicate	2
Protocol	GRO-seq
Library prep	circularization
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	True
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	45 Mb
Stranded gene read counts	TXT file	909 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	2 Mb
dREG bidirectional regions	BED file	556 Kb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	primary cell
Cell type	peritoneal macrophage
Clone/Individual
Strain	C57BL/6
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	Kdo2-Lipid A (100 ng/mL)	-24 hr	0 hr	1 day
treatment	serum starvation (0.5% FBS)	-38 hr	0 hr	38 hr

**Extended notes**
Pipeline info and software versions
Sample notes	Replicate information unclear
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	3
Sample NRO score	2
Raw read length	53
Read depth after trimming	14,278,287
Duplication proportion	0.45014
Proportion reads mapped	0.8448
Exon/intron ratio	3.28957
Unique read proportion	0.5638

**Bidirectionals**
Total # Tfit bidirectionals	18330
# Promoter Tfit bidirectionals	7952
# Intronic Tfit bidirectionals	5618
# Intergenic Tfit bidirectionals	3012
Total # dREG bidirectionals	12883
# Promoter dREG bidirectionals	5793
# Intronic dREG bidirectionals	3454
# Intergenic dREG bidirectionals	2260
Part of Tfit master merge?	True
Part of dREG master merge?	True