DBNascent | Samples

SRZ10004438 Sample ID 185

**Data sources**
Paper identifier	Bahat2019targeting
SRP accession	SRP218860
GEO accession	GSE136024
Samples	SRR10004438
	SRR10004439
	SRR10004440
	SRR10004441

**Technical details**
QC score	2
Replicate	2
Protocol	GRO-seq
Library prep	random primed
Spike-in	None
Single/Paired end	single
Raw strandedness	reverse
Reverse complemented?	True
Mapped strandedness	forward
Timecourse	False
Control/Experimental	control
Wildtype & Untreated?	True
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	392 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	5 Mb
dREG bidirectional regions	BED file	955 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	HeLa
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
vehicle	DMSO	-90 min	0 min	90 min

**Extended notes**
Pipeline info and software versions
Sample notes
Analysis notes	Unstranded - library prep may not have been stranded (unclear in protocol), but good data otherwise

**Sample metrics**
Sample QC score	2
Sample NRO score	3
Raw read length	84
Read depth after trimming	123,610,497
Duplication proportion	0.51893
Proportion reads mapped	0.9241
Exon/intron ratio	3.83974
Unique read proportion	0.61111

**Bidirectionals**
Total # Tfit bidirectionals	60430
# Promoter Tfit bidirectionals	1542
# Intronic Tfit bidirectionals	37986
# Intergenic Tfit bidirectionals	16677
Total # dREG bidirectionals	21908
# Promoter dREG bidirectionals	4015
# Intronic dREG bidirectionals	6901
# Intergenic dREG bidirectionals	9612
Part of Tfit master merge?	False
Part of dREG master merge?	False