DBNascent | Samples

SRZ10004442 Sample ID 186

**Data sources**
Paper identifier	Bahat2019targeting
SRP accession	SRP218860
GEO accession	GSE136024
Samples	SRR10004442
	SRR10004443
	SRR10004444
	SRR10004445

**Technical details**
QC score	2
Replicate	1
Protocol	GRO-seq
Library prep	random primed
Spike-in	None
Single/Paired end	single
Raw strandedness	reverse
Reverse complemented?	True
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	427 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file
dREG bidirectional regions	BED file	1 Mb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	HeLa
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	Spt5-Pol II small-molecule inhibitor 21 (50 microM)	-90 min	0 min	90 min

**Extended notes**
Pipeline info and software versions
Sample notes
Analysis notes	Unstranded - library prep may not have been stranded (unclear in protocol), but good data otherwise

**Sample metrics**
Sample QC score	2
Sample NRO score	2
Raw read length	84
Read depth after trimming	138,476,461
Duplication proportion	0.51537
Proportion reads mapped	0.9079
Exon/intron ratio	3.81862
Unique read proportion	0.6395

**Bidirectionals**
Total # Tfit bidirectionals	None
# Promoter Tfit bidirectionals	None
# Intronic Tfit bidirectionals	None
# Intergenic Tfit bidirectionals	None
Total # dREG bidirectionals	24543
# Promoter dREG bidirectionals	4572
# Intronic dREG bidirectionals	8759
# Intergenic dREG bidirectionals	9802
Part of Tfit master merge?	False
Part of dREG master merge?	False