DBNascent | Samples

SRZ10354620 Sample ID 1051

**Data sources**
Paper identifier	Jaeger2020selective
SRP accession	SRP227189
GEO accession	GSE139462
Samples	SRR10354620
	SRR10354621

**Technical details**
QC score	3
Replicate	1
Protocol	PRO-seq
Library prep	ligation
Spike-in	D. melanogaster
Single/Paired end	single
Raw strandedness	reverse
Reverse complemented?	True
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	144 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	714 Kb
dREG bidirectional regions	BED file	437 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	KBM7
Clone/Individual
Strain
Genotype	Med14 dTAG-inducible degron
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	NVP2 (500 nM)	-30 min	0 min	30 min
treatment	dTAG7 (500 nM)	-2 hr	0 hr	2 hr

**Extended notes**
Pipeline info and software versions
Sample notes	Labeled as experiment MJ-19-30, mentions to only cross-compare within experiment
Analysis notes

**Sample metrics**
Sample QC score	3
Sample NRO score	1
Raw read length	65
Read depth after trimming	85,324,297
Duplication proportion	0.69837
Proportion reads mapped	0.9014
Exon/intron ratio	2.11963
Unique read proportion	0.38465

**Bidirectionals**
Total # Tfit bidirectionals	8174
# Promoter Tfit bidirectionals	4158
# Intronic Tfit bidirectionals	3128
# Intergenic Tfit bidirectionals	713
Total # dREG bidirectionals	10180
# Promoter dREG bidirectionals	5109
# Intronic dREG bidirectionals	3835
# Intergenic dREG bidirectionals	1470
Part of Tfit master merge?	True
Part of dREG master merge?	True