DBNascent | Samples

SRZ10354647 Sample ID 1058

**Data sources**
Paper identifier	Jaeger2020selective
SRP accession	SRP227191
GEO accession	GSE139463
Samples	SRR10354647
	SRR10354648

**Technical details**
QC score	1
Replicate	2
Protocol	TT-seq
Library prep	random primed
Spike-in	SIRV Set 3
Single/Paired end	paired
Raw strandedness	read1 forward
Reverse complemented?	True
Mapped strandedness	read1 forward
Timecourse	False
Control/Experimental	control
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	507 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	1 Mb
dREG bidirectional regions	BED file	974 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	KBM7
Clone/Individual
Strain
Genotype	Med14 dTAG-inducible degron
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	4-thiouridine (500 microM)	-5 min	0 min	5 min
vehicle	DMSO	-2 hr	0 hr	2 hr

**Extended notes**
Pipeline info and software versions
Sample notes	Labeled as experiment MJ-19-1, mentions to only cross-compare within experiments
Analysis notes

**Sample metrics**
Sample QC score	1
Sample NRO score	1
Raw read length	35
Read depth after trimming	113,200,418
Duplication proportion	0.36034
Proportion reads mapped	0.9601
Exon/intron ratio	2.57165
Unique read proportion	0.79479

**Bidirectionals**
Total # Tfit bidirectionals	16834
# Promoter Tfit bidirectionals	5362
# Intronic Tfit bidirectionals	9067
# Intergenic Tfit bidirectionals	1585
Total # dREG bidirectionals	22463
# Promoter dREG bidirectionals	5293
# Intronic dREG bidirectionals	11875
# Intergenic dREG bidirectionals	3877
Part of Tfit master merge?	True
Part of dREG master merge?	True