DBNascent | Samples

SRZ12179474 Sample ID 2889

**Data sources**
Paper identifier	Zhu2021calcium
SRP accession	SRP270993
GEO accession	GSE154051
Samples	SRR12179474
	SRR12179475

**Technical details**
QC score	4
Replicate	1
Protocol	GRO-seq
Library prep	not noted in GEO or publication
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	29 Mb
Stranded gene read counts	TXT file	902 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	484 Kb
dREG bidirectional regions	BED file	204 Kb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	cell line
Cell type	ECOMG
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	A23187 (5 microM)	-4 hr	0 hr	4 hr
treatment	neutrophil differentiation protocol	None	None	None

**Extended notes**
Pipeline info and software versions
Sample notes	No library prep specified, no polyA tails; no replicate info, assume tech reps
Analysis notes

**Sample metrics**
Sample QC score	4
Sample NRO score	2
Raw read length	75
Read depth after trimming	32,165,029
Duplication proportion	0.82604
Proportion reads mapped	0.6861
Exon/intron ratio	3.58759
Unique read proportion	0.19062

**Bidirectionals**
Total # Tfit bidirectionals	5706
# Promoter Tfit bidirectionals	2082
# Intronic Tfit bidirectionals	2122
# Intergenic Tfit bidirectionals	382
Total # dREG bidirectionals	4745
# Promoter dREG bidirectionals	1941
# Intronic dREG bidirectionals	1411
# Intergenic dREG bidirectionals	942
Part of Tfit master merge?	True
Part of dREG master merge?	True