DBNascent | Samples

SRZ1575932 Sample ID 1816

**Data sources**
Paper identifier	Mayer2015native
SRP accession	SRP047174
GEO accession	GSE61332
Samples	SRR1575932
	SRR1575933
	SRR1575934

**Technical details**
QC score	4
Replicate	1
Protocol	NET-seq
Library prep	circularization
Spike-in	None
Single/Paired end	single
Raw strandedness	reverse
Reverse complemented?	True
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	45 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file
dREG bidirectional regions	BED file
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	HeLa S3
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	flavopiridol (1 microM)	-1 hr	0 hr	1 hr

**Extended notes**
Pipeline info and software versions
Sample notes	unique hexamers were ligated first (before circularization) to remove PCR duplicates computationally
Analysis notes

**Sample metrics**
Sample QC score	4
Sample NRO score	3
Raw read length	50
Read depth after trimming	290,953,610
Duplication proportion	0.90435
Proportion reads mapped	0.1801
Exon/intron ratio	5.63396
Unique read proportion	0.12364

**Bidirectionals**
Total # Tfit bidirectionals	None
# Promoter Tfit bidirectionals	None
# Intronic Tfit bidirectionals	None
# Intergenic Tfit bidirectionals	None
Total # dREG bidirectionals	None
# Promoter dREG bidirectionals	None
# Intronic dREG bidirectionals	None
# Intergenic dREG bidirectionals	None
Part of Tfit master merge?	False
Part of dREG master merge?	False