DBNascent | Samples

SRZ1744510 Sample ID 2618

**Data sources**
Paper identifier	Wang2015molecular
SRP	SRP051769
Samples	SRR1744510
	SRR1744511

**Technical details**
QC score	4
Replicate	1
Protocol	GRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Timecourse	True
Control/Experimental	experimental
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	20 Mb
Stranded gene read counts	TXT file	896 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	729 Kb
dREG bidirectional regions	BED file	280 Kb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	cell line
Cell type	ESC
Clone/Individual
Strain
Genotype	Tdg -/-
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	DRB (100 mM)	-3 hr	0 hr	3 hr

**Extended notes**
Pipeline info and software versions
Sample notes
Analysis notes	May needs samtools/1.10 and 60Gb memory for samtools step (that is in my notes but does not match running script, which uses samtools 1.8); One sample required rerun of picardtools; Ran qc redo nextflow

**Sample metrics**
Sample QC score	4
Sample NRO score	1
Raw read length	40
Read depth after trimming	21,326,835
Duplication proportion	0.85168
Proportion reads mapped	0.4631
Exon/intron ratio	1.91054
Unique read proportion	0.14531

**Bidirectionals**
Total # Tfit bidirectionals	8685
# Promoter Tfit bidirectionals	3057
# Intronic Tfit bidirectionals	3130
# Intergenic Tfit bidirectionals	1331
Total # dREG bidirectionals	6528
# Promoter dREG bidirectionals	3289
# Intronic dREG bidirectionals	1614
# Intergenic dREG bidirectionals	1043
Part of Tfit master merge?	True
Part of dREG master merge?	True