DBNascent | Samples

SRZ1745515 Sample ID 698

**Data sources**
Paper identifier	Estaras2015smad
SRP accession	SRP051788
GEO accession	GSE64758
Samples	SRR1745515
	SRR1745516

**Technical details**
QC score	2
Replicate	1
Protocol	GRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	control
Wildtype & Untreated?	True
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	75 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	1 Mb
dREG bidirectional regions	BED file	358 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	ESC
Clone/Individual	H1
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
no treatment		None	None	None

**Extended notes**
Pipeline info and software versions
Sample notes	Labeled (+/- chains) - runs seem to be split in two by lane, thus technical replicates
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	2
Sample NRO score	1
Raw read length	51
Read depth after trimming	36,027,278
Duplication proportion	0.56447
Proportion reads mapped	0.7289
Exon/intron ratio	2.99452
Unique read proportion	0.46933

**Bidirectionals**
Total # Tfit bidirectionals	14003
# Promoter Tfit bidirectionals	4540
# Intronic Tfit bidirectionals	6070
# Intergenic Tfit bidirectionals	2508
Total # dREG bidirectionals	8319
# Promoter dREG bidirectionals	3366
# Intronic dREG bidirectionals	2906
# Intergenic dREG bidirectionals	2041
Part of Tfit master merge?	True
Part of dREG master merge?	True