DBNascent | Samples

SRZ1745519 Sample ID 700

**Data sources**
Paper identifier	Estaras2015smad
SRP accession	SRP051788
GEO accession	GSE64758
Samples	SRR1745519
	SRR1745520

**Technical details**
QC score	2
Replicate	1
Protocol	GRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	66 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	1 Mb
dREG bidirectional regions	BED file	307 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	ESC
Clone/Individual	H1
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	Wnt3a (200 ng/mL)	-6 hr	0 hr	6 hr
treatment	activin A (100 ng/mL)	-6 hr	0 hr	6 hr

**Extended notes**
Pipeline info and software versions
Sample notes	Labeled (+/- chains) - runs seem to be split in two by lane, thus technical replicates
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	2
Sample NRO score	2
Raw read length	51
Read depth after trimming	31,163,597
Duplication proportion	0.59353
Proportion reads mapped	0.7618
Exon/intron ratio	3.42553
Unique read proportion	0.43333

**Bidirectionals**
Total # Tfit bidirectionals	12706
# Promoter Tfit bidirectionals	4194
# Intronic Tfit bidirectionals	5366
# Intergenic Tfit bidirectionals	2222
Total # dREG bidirectionals	7149
# Promoter dREG bidirectionals	3042
# Intronic dREG bidirectionals	2395
# Intergenic dREG bidirectionals	1722
Part of Tfit master merge?	True
Part of dREG master merge?	True