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SRZ1745525 Sample ID 703
| Paper identifier | Estaras2015smad |
| SRP accession | SRP051788 |
| GEO accession | GSE64758 |
| Samples | SRR1745525 |
| SRR1745526 |
| QC score | 2 |
| Replicate | 1 |
| Protocol | GRO-seq |
| Library prep | ligation |
| Spike-in | None |
| Single/Paired end | single |
| Raw strandedness | forward |
| Reverse complemented? | False |
| Mapped strandedness | forward |
| Timecourse | False |
| Control/Experimental | experimental |
| Wildtype & Untreated? | False |
| Outlier | No |
| Unusable | No |
| TDF visualization file | TDF file | |
| Stranded gene read counts | TXT file | |
| Unstranded bidirectional read counts | TXT file | |
| Tfit bidirectional regions | BED file | |
| dREG bidirectional regions | BED file | |
| All data files | ZIP archive | |
| Organism | H. sapiens |
| Sample type | cell line |
| Cell type | ESC |
| Clone/Individual | H1 |
| Strain | |
| Genotype | |
| Construct | siRNA control |
| Condition type | Treatment | Start time | End time | Duration |
| treatment | Wnt3a (200 ng/mL) | -6 hr | 0 hr | 6 hr |
| treatment | activin A (100 ng/mL) | -6 hr | 0 hr | 6 hr |
| Pipeline info and software versions | |
| Sample notes | Labeled (+/- chains) - runs seem to be split in two by lane, thus technical replicates |
| Analysis notes | Ran qc redo nextflow |
| Sample QC score | 2 |
| Sample NRO score | 2 |
| Raw read length | 51 |
| Read depth after trimming | 44,022,177 |
| Duplication proportion | 0.62178 |
| Proportion reads mapped | 0.7517 |
| Exon/intron ratio | 3.07876 |
| Unique read proportion | 0.42065 |
| Total # Tfit bidirectionals | 18255 |
| # Promoter Tfit bidirectionals | 6172 |
| # Intronic Tfit bidirectionals | 7972 |
| # Intergenic Tfit bidirectionals | 3148 |
| Total # dREG bidirectionals | 10610 |
| # Promoter dREG bidirectionals | 4553 |
| # Intronic dREG bidirectionals | 3777 |
| # Intergenic dREG bidirectionals | 2243 |
| Part of Tfit master merge? | True |
| Part of dREG master merge? | True |