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SRZ1745527 Sample ID 704

**Data sources**
Paper identifier	Estaras2015smad
SRP accession	SRP051788
GEO accession	GSE64758
Samples	SRR1745527
	SRR1745528

**Technical details**
QC score	2
Replicate	1
Protocol	GRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	81 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	2 Mb
dREG bidirectional regions	BED file	487 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	ESC
Clone/Individual	H1
Strain
Genotype
Construct	siRNA Yap1

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
no treatment		None	None	None

**Extended notes**
Pipeline info and software versions
Sample notes	Labeled (+/- chains) - runs seem to be split in two by lane, thus technical replicates
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	2
Sample NRO score	2
Raw read length	51
Read depth after trimming	46,292,953
Duplication proportion	0.64752
Proportion reads mapped	0.7366
Exon/intron ratio	3.1924
Unique read proportion	0.39679

**Bidirectionals**
Total # Tfit bidirectionals	19061
# Promoter Tfit bidirectionals	6468
# Intronic Tfit bidirectionals	8515
# Intergenic Tfit bidirectionals	3160
Total # dREG bidirectionals	11311
# Promoter dREG bidirectionals	5062
# Intronic dREG bidirectionals	4029
# Intergenic dREG bidirectionals	2223
Part of Tfit master merge?	True
Part of dREG master merge?	True