DBNascent | Samples

SRZ1951430 Sample ID 951

**Data sources**
Paper identifier	Heinaniemi2016transcription
SRP accession	SRP056832
GEO accession	GSE67540
Samples	SRR1951430
	SRR1951431

**Technical details**
QC score	2
Replicate	2
Protocol	GRO-seq
Library prep	circularization
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	True
Control/Experimental	control
Wildtype & Untreated?	True
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	98 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	2 Mb
dREG bidirectional regions	BED file	877 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	REH
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
no treatment		None	None	None

**Extended notes**
Pipeline info and software versions
Sample notes	Control for 24 hr co-cultures; REH (ACC-20) cell line is ALL leukemia cell line
Analysis notes	Originally downloaded/processed many samples also present in Teppo2016genome - final versions under that ID; Ran qc redo nextflow

**Sample metrics**
Sample QC score	2
Sample NRO score	2
Raw read length	51
Read depth after trimming	35,610,400
Duplication proportion	0.55416
Proportion reads mapped	0.9185
Exon/intron ratio	3.76393
Unique read proportion	0.54935

**Bidirectionals**
Total # Tfit bidirectionals	26427
# Promoter Tfit bidirectionals	11913
# Intronic Tfit bidirectionals	10271
# Intergenic Tfit bidirectionals	4585
Total # dREG bidirectionals	20247
# Promoter dREG bidirectionals	8475
# Intronic dREG bidirectionals	8074
# Intergenic dREG bidirectionals	3878
Part of Tfit master merge?	True
Part of dREG master merge?	True