DBNascent | Samples

SRZ2820485 Sample ID 2644

**Data sources**
Paper identifier	Wei2016long
SRP accession	SRP065285
GEO accession	GSE74356
Samples	SRR2820485
	SRR2820486

**Technical details**
QC score	3
Replicate	3
Protocol	GRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	135 Mb
Stranded gene read counts	TXT file	935 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	2 Mb
dREG bidirectional regions	BED file	1 Mb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	primary cell
Cell type	NSPC
Clone/Individual
Strain	129/Sve
Genotype	Atm -/-
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
no treatment		None	None	None

**Extended notes**
Pipeline info and software versions
Sample notes	triamcinolone acetate-inducible Atm -/- GR-I-SceI 25xI-SceI-c-Myc CRISPR construct induced for 3.5 days with 10 microM triamcinolone acetate
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	3
Sample NRO score	1
Raw read length	51
Read depth after trimming	91,197,236
Duplication proportion	0.75423
Proportion reads mapped	0.8438
Exon/intron ratio	2.23399
Unique read proportion	0.66391

**Bidirectionals**
Total # Tfit bidirectionals	28356
# Promoter Tfit bidirectionals	8798
# Intronic Tfit bidirectionals	10739
# Intergenic Tfit bidirectionals	4619
Total # dREG bidirectionals	25256
# Promoter dREG bidirectionals	6280
# Intronic dREG bidirectionals	9866
# Intergenic dREG bidirectionals	5372
Part of Tfit master merge?	True
Part of dREG master merge?	True