DBNascent | Samples

SRZ2960996 Sample ID 1769

**Data sources**
Paper identifier	Magnuson2015identifying
SRP accession	SRP066673
GEO accession	GSE75398
Samples	SRR2960996
	SRR2960997

**Technical details**
QC score	4
Replicate	1
Protocol	Bru-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	reverse
Reverse complemented?	True
Mapped strandedness	forward
Timecourse	False
Control/Experimental	control
Wildtype & Untreated?	True
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	69 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	393 Kb
dREG bidirectional regions	BED file	318 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	K562
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	bromouridine (2 mM)	-390 min	-360 min	30 min

**Extended notes**
Pipeline info and software versions
Sample notes	Bru-seq; replicate information unclear, but points toward technical replicates
Analysis notes

**Sample metrics**
Sample QC score	4
Sample NRO score	5
Raw read length	52
Read depth after trimming	91,998,094
Duplication proportion	0.9096
Proportion reads mapped	0.9513
Exon/intron ratio	16.8487
Unique read proportion	0.30575

**Bidirectionals**
Total # Tfit bidirectionals	4498
# Promoter Tfit bidirectionals	792
# Intronic Tfit bidirectionals	2263
# Intergenic Tfit bidirectionals	251
Total # dREG bidirectionals	7382
# Promoter dREG bidirectionals	892
# Intronic dREG bidirectionals	4393
# Intergenic dREG bidirectionals	896
Part of Tfit master merge?	True
Part of dREG master merge?	True