SRZ2961008 Sample ID 1775

Data sources
Paper identifier Magnuson2015identifying
SRP accession SRP066673
GEO accession GSE75398
Samples SRR2961008
SRR2961009
SRR2961010
SRR2961011
Technical details
QC score 3
Replicate 2
Protocol Bru-seq
Library prep ligation
Spike-in None
Single/Paired end single
Raw strandedness reverse
Reverse complemented? True
Mapped strandedness forward
Timecourse False
Control/Experimental experimental
Wildtype & Untreated? False
Outlier No
Unusable No
Downloads
TDF visualization file TDF file
Stranded gene read counts TXT file
Unstranded bidirectional read counts TXT file
Tfit bidirectional regions BED file
dREG bidirectional regions BED file
All data files ZIP archive
Cellular details
Organism H. sapiens
Sample type cell line
Cell type HF1
Clone/Individual
Strain
Genotype
Construct
Conditions and treatments
Condition type Treatment Start time End time Duration
treatment bromouridine (2 mM) -30 min 0 min 30 min
treatment ultraviolet C (1 J/m^2/s) -1820 s -1800 s 20 s
Extended notes
Pipeline info and software versions
Sample notes Bru-seq; replicate information unclear, but points toward technical replicates
Analysis notes
Sample metrics
Sample QC score 3
Sample NRO score 3
Raw read length 52
Read depth after trimming 49,309,831
Duplication proportion 0.7034
Proportion reads mapped 0.9582
Exon/intron ratio 5.38209
Unique read proportion 0.38343
Bidirectionals
Total # Tfit bidirectionals 28754
# Promoter Tfit bidirectionals 8317
# Intronic Tfit bidirectionals 14083
# Intergenic Tfit bidirectionals 3585
Total # dREG bidirectionals 14829
# Promoter dREG bidirectionals 4447
# Intronic dREG bidirectionals 6887
# Intergenic dREG bidirectionals 2155
Part of Tfit master merge? True
Part of dREG master merge? True