SRZ2961012 Sample ID 1776

Data sources
Paper identifier Magnuson2015identifying
SRP accession SRP066673
GEO accession GSE75398
Samples SRR2961012
SRR2961013
SRR2961014
SRR2961015
Technical details
QC score 3
Replicate 3
Protocol Bru-seq
Library prep ligation
Spike-in None
Single/Paired end single
Raw strandedness reverse
Reverse complemented? True
Mapped strandedness forward
Timecourse False
Control/Experimental experimental
Wildtype & Untreated? False
Outlier No
Unusable No
Downloads
TDF visualization file TDF file
Stranded gene read counts TXT file
Unstranded bidirectional read counts TXT file
Tfit bidirectional regions BED file
dREG bidirectional regions BED file
All data files ZIP archive
Cellular details
Organism H. sapiens
Sample type cell line
Cell type HF1
Clone/Individual
Strain
Genotype
Construct
Conditions and treatments
Condition type Treatment Start time End time Duration
treatment bromouridine (2 mM) -30 min 0 min 30 min
treatment ultraviolet C (1 J/m^2/s) -1820 s -1800 s 20 s
Extended notes
Pipeline info and software versions
Sample notes Bru-seq; replicate information unclear, but points toward technical replicates
Analysis notes
Sample metrics
Sample QC score 3
Sample NRO score 2
Raw read length 52
Read depth after trimming 57,107,156
Duplication proportion 0.65838
Proportion reads mapped 0.9587
Exon/intron ratio 4.86087
Unique read proportion 0.45998
Bidirectionals
Total # Tfit bidirectionals 29195
# Promoter Tfit bidirectionals 8311
# Intronic Tfit bidirectionals 14261
# Intergenic Tfit bidirectionals 4110
Total # dREG bidirectionals 16726
# Promoter dREG bidirectionals 4733
# Intronic dREG bidirectionals 7972
# Intergenic dREG bidirectionals 2728
Part of Tfit master merge? True
Part of dREG master merge? True