DBNascent | Samples

SRZ354100 Sample ID 1824

**Data sources**
Paper identifier	Mckinlay2011genome
SRP accession	SRP009043
GEO accession	GSE33136
Samples	SRR354100
	SRR354101
	SRR354102

**Technical details**
QC score	5
Replicate	3
Protocol	PRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	2 Mb
Stranded gene read counts	TXT file
Unstranded bidirectional read counts	TXT file
Tfit bidirectional regions	BED file
dREG bidirectional regions	BED file
All data files	ZIP archive

**Cellular details**
Organism	S. cerevisiae
Sample type	organism
Cell type	whole yeast
Clone/Individual
Strain	BY4742
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	heat shock (39 Celsius)	-20 min	0 min	20 min

**Extended notes**
Pipeline info and software versions
Sample notes	States that this is a 5' nuclear run-on RNA library, but fragmented before ligation
Analysis notes

**Sample metrics**
Sample QC score	5
Sample NRO score	1
Raw read length	36
Read depth after trimming	4,813,921
Duplication proportion	0.98555
Proportion reads mapped	0.6822
Exon/intron ratio	0.54508
Unique read proportion	0.00647

**Bidirectionals**
Total # Tfit bidirectionals	None
# Promoter Tfit bidirectionals	None
# Intronic Tfit bidirectionals	None
# Intergenic Tfit bidirectionals	None
Total # dREG bidirectionals	None
# Promoter dREG bidirectionals	None
# Intronic dREG bidirectionals	None
# Intergenic dREG bidirectionals	None
Part of Tfit master merge?	False
Part of dREG master merge?	False