DBNascent | Samples

SRZ3734610 Sample ID 671

**Data sources**
Paper identifier	Emmett2017histone
SRP accession	SRP077663
GEO accession	GSE83926
Samples	SRR3734610
	SRR3734611

**Technical details**
QC score	3
Replicate	1
Protocol	GRO-seq
Library prep	circularization
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	261 Mb
Stranded gene read counts	TXT file	936 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	3 Mb
dREG bidirectional regions	BED file	2 Mb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	tissue
Cell type	brown adipose tissue
Clone/Individual
Strain
Genotype	Hdac3 fl/fl
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	thermoneutrality (29 Celsius)	-14 day	0 day	14 day

**Extended notes**
Pipeline info and software versions
Sample notes	Doesn't mention circularizing, but does mention polyA-tailing
Analysis notes

**Sample metrics**
Sample QC score	3
Sample NRO score	1
Raw read length	50
Read depth after trimming	242,424,254
Duplication proportion	0.66875
Proportion reads mapped	0.4849
Exon/intron ratio	2.90465
Unique read proportion	0.56897

**Bidirectionals**
Total # Tfit bidirectionals	33319
# Promoter Tfit bidirectionals	13340
# Intronic Tfit bidirectionals	9697
# Intergenic Tfit bidirectionals	8415
Total # dREG bidirectionals	43869
# Promoter dREG bidirectionals	9591
# Intronic dREG bidirectionals	19022
# Intergenic dREG bidirectionals	12512
Part of Tfit master merge?	True
Part of dREG master merge?	True