DBNascent | Samples

SRZ3734612 Sample ID 672

**Data sources**
Paper identifier	Emmett2017histone
SRP accession	SRP077663
GEO accession	GSE83926
Samples	SRR3734612
	SRR3734613

**Technical details**
QC score	4
Replicate	1
Protocol	GRO-seq
Library prep	circularization
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	103 Mb
Stranded gene read counts	TXT file	925 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	2 Mb
dREG bidirectional regions	BED file	1 Mb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	tissue
Cell type	brown adipose tissue
Clone/Individual
Strain
Genotype	Hdac3 fl/fl; adiponectin-Cre
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	thermoneutrality (29 Celsius)	-14 day	0 day	14 day

**Extended notes**
Pipeline info and software versions
Sample notes	Doesn't mention circularizing, but does mention polyA-tailing
Analysis notes

**Sample metrics**
Sample QC score	4
Sample NRO score	1
Raw read length	50
Read depth after trimming	232,399,446
Duplication proportion	0.83913
Proportion reads mapped	0.4076
Exon/intron ratio	1.26647
Unique read proportion	0.24713

**Bidirectionals**
Total # Tfit bidirectionals	24913
# Promoter Tfit bidirectionals	10182
# Intronic Tfit bidirectionals	7847
# Intergenic Tfit bidirectionals	5023
Total # dREG bidirectionals	26882
# Promoter dREG bidirectionals	9206
# Intronic dREG bidirectionals	10048
# Intergenic dREG bidirectionals	5917
Part of Tfit master merge?	True
Part of dREG master merge?	True