DBNascent | Samples

SRZ3734614 Sample ID 673

**Data sources**
Paper identifier	Emmett2017histone
SRP accession	SRP077663
GEO accession	GSE83926
Samples	SRR3734614
	SRR3734615
	SRR3734616

**Technical details**
QC score	2
Replicate	1
Protocol	GRO-seq
Library prep	circularization
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	control
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	403 Mb
Stranded gene read counts	TXT file	939 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	3 Mb
dREG bidirectional regions	BED file	2 Mb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	tissue
Cell type	brown adipose tissue
Clone/Individual
Strain
Genotype	Hdac3 fl/fl
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
no treatment		None	None	None

**Extended notes**
Pipeline info and software versions
Sample notes	Kept at room temp (22 Celsius); Doesn't mention circularizing, but does mention polyA-tailing
Analysis notes

**Sample metrics**
Sample QC score	2
Sample NRO score	1
Raw read length	50
Read depth after trimming	275,569,705
Duplication proportion	0.59488
Proportion reads mapped	0.5641
Exon/intron ratio	2.61426
Unique read proportion	0.67697

**Bidirectionals**
Total # Tfit bidirectionals	34663
# Promoter Tfit bidirectionals	13831
# Intronic Tfit bidirectionals	9560
# Intergenic Tfit bidirectionals	9259
Total # dREG bidirectionals	50333
# Promoter dREG bidirectionals	8541
# Intronic dREG bidirectionals	22313
# Intergenic dREG bidirectionals	15969
Part of Tfit master merge?	True
Part of dREG master merge?	True