DBNascent | Samples

SRZ4012400 Sample ID 590

**Data sources**
Paper identifier	Danko2018dynamic
SRP accession	SRP081110
GEO accession	GSE85337
Samples	SRR4012400
	SRR4012401

**Technical details**
QC score	3
Replicate	3
Protocol	PRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	reverse
Reverse complemented?	True
Mapped strandedness	forward
Timecourse	False
Control/Experimental	control
Wildtype & Untreated?	True
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	35 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	1 Mb
dREG bidirectional regions	BED file	536 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	primary cell
Cell type	CD4+ T cell
Clone/Individual	individual 3
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
vehicle	ethanol	-30 min	0 min	30 min
vehicle	DMSO	-30 min	0 min	30 min

**Extended notes**
Pipeline info and software versions
Sample notes	References Kwak2013precise for library prep, no specifics in paper or GEO
Analysis notes

**Sample metrics**
Sample QC score	3
Sample NRO score	3
Raw read length	51
Read depth after trimming	16,263,064
Duplication proportion	0.75617
Proportion reads mapped	0.8229
Exon/intron ratio	5.01931
Unique read proportion	0.29171

**Bidirectionals**
Total # Tfit bidirectionals	15660
# Promoter Tfit bidirectionals	7405
# Intronic Tfit bidirectionals	6169
# Intergenic Tfit bidirectionals	1773
Total # dREG bidirectionals	12432
# Promoter dREG bidirectionals	6873
# Intronic dREG bidirectionals	4222
# Intergenic dREG bidirectionals	1363
Part of Tfit master merge?	True
Part of dREG master merge?	True