DBNascent | Samples

SRZ4012404 Sample ID 592

**Data sources**
Paper identifier	Danko2018dynamic
SRP accession	SRP081110
GEO accession	GSE85337
Samples	SRR4012404
	SRR4012405

**Technical details**
QC score	3
Replicate	1
Protocol	PRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	reverse
Reverse complemented?	True
Mapped strandedness	forward
Timecourse	False
Control/Experimental	control
Wildtype & Untreated?	True
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	110 Mb
Stranded gene read counts	TXT file
Unstranded bidirectional read counts	TXT file
Tfit bidirectional regions	BED file
dREG bidirectional regions	BED file
All data files	ZIP archive

**Cellular details**
Organism	P. troglodytes
Sample type	primary cell
Cell type	CD4+ T cell
Clone/Individual	individual 3
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
vehicle	ethanol	-30 min	0 min	30 min
vehicle	DMSO	-30 min	0 min	30 min

**Extended notes**
Pipeline info and software versions
Sample notes	References Kwak2013precise for library prep, no specifics in paper or GEO
Analysis notes

**Sample metrics**
Sample QC score	3
Sample NRO score	2
Raw read length	50
Read depth after trimming	49,135,905
Duplication proportion	0.72233
Proportion reads mapped	0.8119
Exon/intron ratio	4.66709
Unique read proportion	0.50113

**Bidirectionals**
Total # Tfit bidirectionals	None
# Promoter Tfit bidirectionals	None
# Intronic Tfit bidirectionals	None
# Intergenic Tfit bidirectionals	None
Total # dREG bidirectionals	None
# Promoter dREG bidirectionals	None
# Intronic dREG bidirectionals	None
# Intergenic dREG bidirectionals	None
Part of Tfit master merge?	True
Part of dREG master merge?	True