SRZ4012415 Sample ID 596

Data sources
Paper identifier Danko2018dynamic
SRP accession SRP081110
GEO accession GSE85337
Samples SRR4012415
SRR4012416
Technical details
QC score 4
Replicate 4
Protocol PRO-seq
Library prep ligation
Spike-in None
Single/Paired end single
Raw strandedness reverse
Reverse complemented? True
Mapped strandedness forward
Timecourse False
Control/Experimental control
Wildtype & Untreated? True
Outlier No
Unusable No
Downloads
TDF visualization file TDF file 269 Mb
Cellular details
Organism M. mulatta
Sample type primary cell
Cell type CD4+ T cell
Clone/Individual individual 1
Strain
Genotype
Construct
Conditions and treatments
Condition type Treatment Start time End time Duration
vehicle ethanol -30 min 0 min 30 min
vehicle DMSO -30 min 0 min 30 min
Extended notes
Pipeline info and software versions
Sample notes Individual 1, but no corresponding treatment - wanted to keep reps from same individual consistent, so made these rep4; References Kwak2013precise for library prep, no specifics in paper or GEO
Analysis notes
Sample metrics
Sample QC score 4
Sample NRO score 2
Raw read length 51
Read depth after trimming 10,342,444
Duplication proportion 0.60709
Proportion reads mapped 0.7665
Exon/intron ratio 3.88153
Unique read proportion 0.34136
Bidirectionals
Total # Tfit bidirectionals None
# Promoter Tfit bidirectionals None
# Intronic Tfit bidirectionals None
# Intergenic Tfit bidirectionals None
Total # dREG bidirectionals None
# Promoter dREG bidirectionals None
# Intronic dREG bidirectionals None
# Intergenic dREG bidirectionals None
Part of Tfit master merge? True
Part of dREG master merge? True