SRZ4012420 Sample ID 598

Data sources
Paper identifier Danko2018dynamic
SRP accession SRP081110
GEO accession GSE85337
Samples SRR4012420
SRR4012421
SRR4012422
Technical details
QC score 4
Replicate 1
Protocol PRO-seq
Library prep ligation
Spike-in None
Single/Paired end single
Raw strandedness reverse
Reverse complemented? True
Mapped strandedness forward
Timecourse False
Control/Experimental experimental
Wildtype & Untreated? False
Outlier No
Unusable No
Downloads
TDF visualization file TDF file 389 Mb
Cellular details
Organism M. mulatta
Sample type primary cell
Cell type CD4+ T cell
Clone/Individual individual 2
Strain
Genotype
Construct
Conditions and treatments
Condition type Treatment Start time End time Duration
treatment ionomycin (1 mM) -30 min 0 min 30 min
treatment phorbol 12-myristate 13-acetate (25 ng/mL) -30 min 0 min 30 min
Extended notes
Pipeline info and software versions
Sample notes References Kwak2013precise for library prep, no specifics in paper or GEO
Analysis notes
Sample metrics
Sample QC score 4
Sample NRO score 2
Raw read length 50
Read depth after trimming 57,762,688
Duplication proportion 0.84016
Proportion reads mapped 0.8121
Exon/intron ratio 3.83259
Unique read proportion 0.34137
Bidirectionals
Total # Tfit bidirectionals None
# Promoter Tfit bidirectionals None
# Intronic Tfit bidirectionals None
# Intergenic Tfit bidirectionals None
Total # dREG bidirectionals None
# Promoter dREG bidirectionals None
# Intronic dREG bidirectionals None
# Intergenic dREG bidirectionals None
Part of Tfit master merge? True
Part of dREG master merge? True