SRZ4012426 Sample ID 600

Data sources
Paper identifier Danko2018dynamic
SRP accession SRP081110
GEO accession GSE85337
Samples SRR4012426
SRR4012427
SRR4012428
Technical details
QC score 3
Replicate 2
Protocol PRO-seq
Library prep ligation
Spike-in None
Single/Paired end single
Raw strandedness reverse
Reverse complemented? True
Mapped strandedness forward
Timecourse False
Control/Experimental experimental
Wildtype & Untreated? False
Outlier No
Unusable No
Downloads
TDF visualization file TDF file 521 Mb
Cellular details
Organism M. mulatta
Sample type primary cell
Cell type CD4+ T cell
Clone/Individual individual 3
Strain
Genotype
Construct
Conditions and treatments
Condition type Treatment Start time End time Duration
treatment ionomycin (1 mM) -30 min 0 min 30 min
treatment phorbol 12-myristate 13-acetate (25 ng/mL) -30 min 0 min 30 min
Extended notes
Pipeline info and software versions
Sample notes References Kwak2013precise for library prep, no specifics in paper or GEO
Analysis notes
Sample metrics
Sample QC score 3
Sample NRO score 1
Raw read length 51
Read depth after trimming 55,010,357
Duplication proportion 0.74047
Proportion reads mapped 0.7994
Exon/intron ratio 2.74224
Unique read proportion 0.49252
Bidirectionals
Total # Tfit bidirectionals None
# Promoter Tfit bidirectionals None
# Intronic Tfit bidirectionals None
# Intergenic Tfit bidirectionals None
Total # dREG bidirectionals None
# Promoter dREG bidirectionals None
# Intronic dREG bidirectionals None
# Intergenic dREG bidirectionals None
Part of Tfit master merge? True
Part of dREG master merge? True