DBNascent | Samples

SRZ5298787 Sample ID 1020

**Data sources**
Paper identifier	Incarnato2017vivo
SRP accession	SRP100931
GEO accession	GSE95567
Samples	SRR5298787
	SRR5298788
	SRR5298789
	SRR5298790

**Technical details**
QC score	4
Replicate	2
Protocol	other
Library prep	ligation
Spike-in	None
Single/Paired end	paired
Raw strandedness	read1 forward
Reverse complemented?	True
Mapped strandedness	read1 forward
Timecourse	False
Control/Experimental	control
Wildtype & Untreated?	True
Outlier	No
Unusable	Yes

**Downloads**
TDF visualization file	TDF file	27 Mb
Stranded gene read counts	TXT file
Unstranded bidirectional read counts	TXT file
Tfit bidirectional regions	BED file
dREG bidirectional regions	BED file
All data files	ZIP archive

**Cellular details**
Organism	E. coli
Sample type	organism
Cell type	whole bacteria
Clone/Individual
Strain	K-12
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
no treatment		None	None	None

**Extended notes**
Pipeline info and software versions
Sample notes	SPET-seq (Ribo- RNA); This is structural data from in vitro nascent transcription - not useful for comparison purposes
Analysis notes	Preseq failed for SRZ

**Sample metrics**
Sample QC score	4
Sample NRO score	1
Raw read length	76
Read depth after trimming	232,904,591
Duplication proportion	0.79627
Proportion reads mapped	0.8854
Exon/intron ratio	2.51323
Unique read proportion	0.12638

**Bidirectionals**
Total # Tfit bidirectionals	None
# Promoter Tfit bidirectionals	None
# Intronic Tfit bidirectionals	None
# Intergenic Tfit bidirectionals	None
Total # dREG bidirectionals	None
# Promoter dREG bidirectionals	None
# Intronic dREG bidirectionals	None
# Intergenic dREG bidirectionals	None
Part of Tfit master merge?	False
Part of dREG master merge?	False