DBNascent | Samples

SRZ579347 Sample ID 571

**Data sources**
Paper identifier	Danko2013signaling
SRP accession	SRP015988
GEO accession	GSE41324
Samples	SRR579347
	SRR579348
	SRR579349
	SRR579350
	SRR579351
	SRR579352

**Technical details**
QC score	2
Replicate	1
Protocol	GRO-seq
Library prep	circularization
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	True
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	68 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	2 Mb
dREG bidirectional regions	BED file	825 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	MCF7
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	17-beta-estradiol (100 nM)	-25 min	0 min	25 min

**Extended notes**
Pipeline info and software versions
Sample notes
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	2
Sample NRO score	2
Raw read length	50
Read depth after trimming	19,255,335
Duplication proportion	0.44662
Proportion reads mapped	0.8217
Exon/intron ratio	3.166
Unique read proportion	0.59801

**Bidirectionals**
Total # Tfit bidirectionals	26390
# Promoter Tfit bidirectionals	12210
# Intronic Tfit bidirectionals	10017
# Intergenic Tfit bidirectionals	4376
Total # dREG bidirectionals	18979
# Promoter dREG bidirectionals	9201
# Intronic dREG bidirectionals	7023
# Intergenic dREG bidirectionals	2960
Part of Tfit master merge?	True
Part of dREG master merge?	True