DBNascent | Samples

SRZ579353 Sample ID 572

**Data sources**
Paper identifier	Danko2013signaling
SRP accession	SRP015988
GEO accession	GSE41324
Samples	SRR579353
	SRR579354
	SRR579355
	SRR579356
	SRR579357
	SRR579358
	SRR579359

**Technical details**
QC score	1
Replicate	2
Protocol	GRO-seq
Library prep	circularization
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	True
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	79 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	3 Mb
dREG bidirectional regions	BED file	853 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	MCF7
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	17-beta-estradiol (100 nM)	-25 min	0 min	25 min

**Extended notes**
Pipeline info and software versions
Sample notes
Analysis notes	Ran qc redo nextflow; preseq didn't work on SRR579356 before concatenation due to low read depth

**Sample metrics**
Sample QC score	1
Sample NRO score	2
Raw read length	50
Read depth after trimming	23,258,177
Duplication proportion	0.4966
Proportion reads mapped	0.8363
Exon/intron ratio	3.23701
Unique read proportion	0.59599

**Bidirectionals**
Total # Tfit bidirectionals	29553
# Promoter Tfit bidirectionals	13048
# Intronic Tfit bidirectionals	11436
# Intergenic Tfit bidirectionals	5102
Total # dREG bidirectionals	19606
# Promoter dREG bidirectionals	9089
# Intronic dREG bidirectionals	7343
# Intergenic dREG bidirectionals	3228
Part of Tfit master merge?	True
Part of dREG master merge?	True