DBNascent | Samples

SRZ5861774 Sample ID 1250

**Data sources**
Paper identifier	Kourtis2018oncogenic
SRP accession	SRP113457
GEO accession	GSE101803
Samples	SRR5861774
	SRR5861775

**Technical details**
QC score	4
Replicate	2
Protocol	GRO-seq
Library prep	not noted in GEO or publication
Spike-in	None
Single/Paired end	single
Raw strandedness	reverse
Reverse complemented?	True
Mapped strandedness	forward
Timecourse	True
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	39 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	927 Kb
dREG bidirectional regions	BED file	212 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	CUTLL1
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	gamma-secretase inhibitor (1 microM)	-4360 min	-40 min	3 day

**Extended notes**
Pipeline info and software versions
Sample notes	gamma-secretase inhibitor is Compound E, Alexis Biochemicals
Analysis notes

**Sample metrics**
Sample QC score	4
Sample NRO score	2
Raw read length	51
Read depth after trimming	8,780,830
Duplication proportion	0.38367
Proportion reads mapped	0.9549
Exon/intron ratio	3.34899
Unique read proportion	0.60151

**Bidirectionals**
Total # Tfit bidirectionals	10637
# Promoter Tfit bidirectionals	4143
# Intronic Tfit bidirectionals	4498
# Intergenic Tfit bidirectionals	1154
Total # dREG bidirectionals	4933
# Promoter dREG bidirectionals	2682
# Intronic dREG bidirectionals	1615
# Intergenic dREG bidirectionals	610
Part of Tfit master merge?	True
Part of dREG master merge?	True