DBNascent | Samples

SRZ5861780 Sample ID 1253

**Data sources**
Paper identifier	Kourtis2018oncogenic
SRP accession	SRP113457
GEO accession	GSE101803
Samples	SRR5861780
	SRR5861781

**Technical details**
QC score	3
Replicate	2
Protocol	GRO-seq
Library prep	not noted in GEO or publication
Spike-in	None
Single/Paired end	single
Raw strandedness	reverse
Reverse complemented?	True
Mapped strandedness	forward
Timecourse	True
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	46 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	1 Mb
dREG bidirectional regions	BED file	298 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	CUTLL1
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	gamma-secretase inhibitor (1 microM)	-4400 min	-80 min	3 day

**Extended notes**
Pipeline info and software versions
Sample notes	gamma-secretase inhibitor is Compound E, Alexis Biochemicals
Analysis notes

**Sample metrics**
Sample QC score	3
Sample NRO score	2
Raw read length	51
Read depth after trimming	11,253,150
Duplication proportion	0.39474
Proportion reads mapped	0.9561
Exon/intron ratio	3.58491
Unique read proportion	0.60887

**Bidirectionals**
Total # Tfit bidirectionals	13448
# Promoter Tfit bidirectionals	5344
# Intronic Tfit bidirectionals	5571
# Intergenic Tfit bidirectionals	1693
Total # dREG bidirectionals	6910
# Promoter dREG bidirectionals	3694
# Intronic dREG bidirectionals	2241
# Intergenic dREG bidirectionals	963
Part of Tfit master merge?	True
Part of dREG master merge?	True