DBNascent | Samples

SRZ5944050 Sample ID 2110

**Data sources**
Paper identifier	Rahnamoun2017mutant
SRP accession	SRP115797
GEO accession	GSE102796
Samples	SRR5944050
	SRR5944052

**Technical details**
QC score	1
Replicate	2
Protocol	GRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	93 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	3 Mb
dREG bidirectional regions	BED file	906 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	SW480
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	TNF-alpha (12.5 ng/mL)	-16 hr	0 hr	16 hr

**Extended notes**
Pipeline info and software versions
Sample notes	Replicate information is a guess - states 2 biological replicates, but no idea which SRRs are tech reps of each other
Analysis notes

**Sample metrics**
Sample QC score	1
Sample NRO score	1
Raw read length	50
Read depth after trimming	29,680,667
Duplication proportion	0.40035
Proportion reads mapped	0.8411
Exon/intron ratio	2.79353
Unique read proportion	0.62951

**Bidirectionals**
Total # Tfit bidirectionals	32857
# Promoter Tfit bidirectionals	12421
# Intronic Tfit bidirectionals	13358
# Intergenic Tfit bidirectionals	6402
Total # dREG bidirectionals	20920
# Promoter dREG bidirectionals	8302
# Intronic dREG bidirectionals	7852
# Intergenic dREG bidirectionals	4725
Part of Tfit master merge?	True
Part of dREG master merge?	True