DBNascent | Samples

SRZ5944951 Sample ID 881

**Data sources**
Paper identifier	Gao2017thyroid
SRP accession	SRP115827
GEO accession	GSE102819
Samples	SRR5944951
	SRR5944953

**Technical details**
QC score	4
Replicate	2
Protocol	GRO-seq
Library prep	circularization
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	34 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	400 Kb
dREG bidirectional regions	BED file	117 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	primary cell
Cell type	CD34+ erythroblast
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	GC-1 (1 M)	-6 hr	0 hr	6 hr

**Extended notes**
Pipeline info and software versions
Sample notes	No clear replicate information - paper makes no mention of biological replicates for GRO-Seq but does for RNA-Seq, so these may be technical replicates
Analysis notes

**Sample metrics**
Sample QC score	4
Sample NRO score	2
Raw read length	50
Read depth after trimming	50,590,167
Duplication proportion	0.91235
Proportion reads mapped	0.7686
Exon/intron ratio	3.49655
Unique read proportion	0.11784

**Bidirectionals**
Total # Tfit bidirectionals	4600
# Promoter Tfit bidirectionals	2343
# Intronic Tfit bidirectionals	1632
# Intergenic Tfit bidirectionals	167
Total # dREG bidirectionals	2714
# Promoter dREG bidirectionals	1701
# Intronic dREG bidirectionals	800
# Intergenic dREG bidirectionals	373
Part of Tfit master merge?	True
Part of dREG master merge?	True