DBNascent | Samples

SRZ6290543 Sample ID 2122

**Data sources**
Paper identifier	Rao2017cohesin
SRP accession	SRP124968
GEO accession	GSE106886
Samples	SRR6290543
	SRR6290544
	SRR6290545
	SRR6290546

**Technical details**
QC score	1
Replicate	2
Protocol	PRO-seq
Library prep	ligation
Spike-in	D. melanogaster
Single/Paired end	paired
Raw strandedness	read1 reverse
Reverse complemented?	False
Mapped strandedness	read1 reverse
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	310 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	3 Mb
dREG bidirectional regions	BED file	1 Mb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	HCT116
Clone/Individual
Strain
Genotype	Rad21 auxin-inducible degron
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	indoleacetic acid (500 microM)	-6 hr	0 hr	6 hr

**Extended notes**
Pipeline info and software versions
Sample notes	Library prep not noted in paper, but cites Engreitz2016local
Analysis notes

**Sample metrics**
Sample QC score	1
Sample NRO score	2
Raw read length	30
Read depth after trimming	39,506,234
Duplication proportion	0.10814
Proportion reads mapped	0.9169
Exon/intron ratio	3.78747
Unique read proportion	0.87495

**Bidirectionals**
Total # Tfit bidirectionals	33506
# Promoter Tfit bidirectionals	14485
# Intronic Tfit bidirectionals	11842
# Intergenic Tfit bidirectionals	7665
Total # dREG bidirectionals	27887
# Promoter dREG bidirectionals	7527
# Intronic dREG bidirectionals	11844
# Intergenic dREG bidirectionals	6837
Part of Tfit master merge?	True
Part of dREG master merge?	True