DBNascent | Samples

SRZ6427123 Sample ID 462

**Data sources**
Paper identifier	Chen2018rna
SRP accession	SRP099055
GEO accession	GSE94662
Samples	SRR6427123
	SRR6427124

**Technical details**
QC score	4
Replicate	1
Protocol	GRO-seq
Library prep	circularization
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	22 Mb
Stranded gene read counts	TXT file	903 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	381 Kb
dREG bidirectional regions	BED file	110 Kb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	primary cell
Cell type	splenic B cell
Clone/Individual
Strain
Genotype
Construct	shRNA Rod1

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	lipopolysaccharide (5 microg/mL)	-3 day	0 day	3 day

**Extended notes**
Pipeline info and software versions
Sample notes	Biological replicates clearly labeled, assumed two runs per bio rep were technical replicates
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	4
Sample NRO score	1
Raw read length	52
Read depth after trimming	54,637,484
Duplication proportion	0.93043
Proportion reads mapped	0.6047
Exon/intron ratio	1.87903
Unique read proportion	0.08366

**Bidirectionals**
Total # Tfit bidirectionals	4560
# Promoter Tfit bidirectionals	1626
# Intronic Tfit bidirectionals	1300
# Intergenic Tfit bidirectionals	85
Total # dREG bidirectionals	2560
# Promoter dREG bidirectionals	1207
# Intronic dREG bidirectionals	566
# Intergenic dREG bidirectionals	475
Part of Tfit master merge?	True
Part of dREG master merge?	True