DBNascent | Samples

SRZ6427125 Sample ID 463

**Data sources**
Paper identifier	Chen2018rna
SRP accession	SRP099055
GEO accession	GSE94662
Samples	SRR6427125
	SRR6427126

**Technical details**
QC score	4
Replicate	2
Protocol	GRO-seq
Library prep	circularization
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	27 Mb
Stranded gene read counts	TXT file	905 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	506 Kb
dREG bidirectional regions	BED file	162 Kb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	primary cell
Cell type	splenic B cell
Clone/Individual
Strain
Genotype
Construct	shRNA Rod1

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	lipopolysaccharide (5 microg/mL)	-3 day	0 day	3 day

**Extended notes**
Pipeline info and software versions
Sample notes	Biological replicates clearly labeled, assumed two runs per bio rep were technical replicates
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	4
Sample NRO score	1
Raw read length	52
Read depth after trimming	72,438,479
Duplication proportion	0.92904
Proportion reads mapped	0.5837
Exon/intron ratio	1.90124
Unique read proportion	0.08672

**Bidirectionals**
Total # Tfit bidirectionals	6039
# Promoter Tfit bidirectionals	2275
# Intronic Tfit bidirectionals	1709
# Intergenic Tfit bidirectionals	113
Total # dREG bidirectionals	3767
# Promoter dREG bidirectionals	1865
# Intronic dREG bidirectionals	789
# Intergenic dREG bidirectionals	627
Part of Tfit master merge?	True
Part of dREG master merge?	True