DBNascent | Samples

SRZ6427127 Sample ID 464

**Data sources**
Paper identifier	Chen2018rna
SRP accession	SRP099055
GEO accession	GSE94662
Samples	SRR6427127
	SRR6427128

**Technical details**
QC score	5
Replicate	1
Protocol	GRO-seq
Library prep	circularization
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	control
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	20 Mb
Stranded gene read counts	TXT file	902 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	387 Kb
dREG bidirectional regions	BED file	114 Kb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	primary cell
Cell type	splenic B cell
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	lipopolysaccharide (5 microg/mL)	-3 day	0 day	3 day

**Extended notes**
Pipeline info and software versions
Sample notes	Biological replicates clearly labeled, assumed two runs per bio rep were technical replicates
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	5
Sample NRO score	1
Raw read length	52
Read depth after trimming	74,005,814
Duplication proportion	0.95601
Proportion reads mapped	0.5705
Exon/intron ratio	1.26363
Unique read proportion	0.05566

**Bidirectionals**
Total # Tfit bidirectionals	4641
# Promoter Tfit bidirectionals	1850
# Intronic Tfit bidirectionals	1263
# Intergenic Tfit bidirectionals	87
Total # dREG bidirectionals	2648
# Promoter dREG bidirectionals	1363
# Intronic dREG bidirectionals	544
# Intergenic dREG bidirectionals	339
Part of Tfit master merge?	True
Part of dREG master merge?	True