DBNascent | Samples

SRZ6427129 Sample ID 465

**Data sources**
Paper identifier	Chen2018rna
SRP accession	SRP099055
GEO accession	GSE94662
Samples	SRR6427129
	SRR6427130

**Technical details**
QC score	5
Replicate	2
Protocol	GRO-seq
Library prep	circularization
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	control
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	20 Mb
Stranded gene read counts	TXT file	902 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	386 Kb
dREG bidirectional regions	BED file	115 Kb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	primary cell
Cell type	splenic B cell
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	lipopolysaccharide (5 microg/mL)	-3 day	0 day	3 day

**Extended notes**
Pipeline info and software versions
Sample notes	Biological replicates clearly labeled, assumed two runs per bio rep were technical replicates
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	5
Sample NRO score	1
Raw read length	52
Read depth after trimming	72,562,824
Duplication proportion	0.95383
Proportion reads mapped	0.5659
Exon/intron ratio	1.31361
Unique read proportion	0.05852

**Bidirectionals**
Total # Tfit bidirectionals	4626
# Promoter Tfit bidirectionals	1850
# Intronic Tfit bidirectionals	1289
# Intergenic Tfit bidirectionals	84
Total # dREG bidirectionals	2662
# Promoter dREG bidirectionals	1396
# Intronic dREG bidirectionals	556
# Intergenic dREG bidirectionals	364
Part of Tfit master merge?	True
Part of dREG master merge?	True